reck (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc reck
Reck, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reck/TIMP1+Rabbit+mAb/pm41603922-46-69-70
Average 95 stars, based on 96 article reviews

reck - by Bioz Stars, 2026-09

95/100 stars

Images

Staining:

Article Title: Repurposing ramipril to mitigate EMT-like transition in endometriosis by PI3K/AKT/S6K1 signalling pathway: a study in endometriosis induced rats.
Article Snippet: Endometriosis, an atypical benign disorder, may disrupt epithelial-mesenchymal transition (EMT) due to a dysregulated balance between matrix metalloproteinases (MMPs) and their inhibitors.. Ramipril, an angiotensin converting enzyme (ACE) inhibitor, is crucial in mediating angiogenesis, inflammation, oxidative stress, and apoptosis.. In the current work, we investigated how ramipril modulates EMT in endometriosis rats.

Article Title: Supporting Information (SI) 1 Multi-Functional Nanoparticles Facilitate Molecular Targeting and miRNA Delivery to Inhibit Atherosclerosis in ApoE Mice
Article Snippet: .. Immunohistochemical staining was carried out using following antibodies: TIMP3 (Abcam) (1:100) and RECK (cell signaling) (1:100) overnight at 4°C. .. Secondary staining for TIMP3 and RECK was performed using DyLight 549- conjugated donkey anti-rabbit secondary antibody (Jackson Immunoresearch) (1:250).

Radio Immunoprecipitation:

Saline:

other:

Article Title: Empagliflozin Reverses Oxidized LDL-Induced RECK Suppression, Cardiotrophin-1 Expression, MMP Activation, and Human Aortic Smooth Muscle Cell Proliferation and Migration.
Article Snippet: The following primary antibodies were used: RECK (1 : 1,000; catalog# 3433, Cell Signaling Technology, Inc./CST), α-Tubulin (1 : 1,000; #2144, CST), NF-κB p65 (1 : 1,000; #3033; CST), MMP2 (1 : 500; ab97779, Abcam), MMP9 (1 : 1,000; #2270, CST), polyclonal human LIFR (used in neutralization (10 μg/ml) and western blotting (0.1 μg/ml)), #AF-249-NA, R&D Systems, Minneapolis, MN), and polyclonal antihuman gp130 (used in neutralization (2.5 μg/ml) and Western blotting (1 μg/ml)), #AF-228-NA, R&D Systems, Minneapolis,MN), control IgG (normal goat IgG control, #AF108-C, R&D Systems).

Immunohistochemical staining:

Bioprocessing:

Article Title: Complex I Modulator BI4500 Reduces MASH by Limiting Oxidative Stress and Reprogramming Lipid Metabolism via AMPK in MCD Rats
Article Snippet: .. Primary Antibody Secondary Antibody Rabbit monoclonal antibodies p-ACC (Cell Signaling Technology) 1:1000 1:2000 ACC (Cell Signaling Technology) 1:1000 1:2000 p-AMPK (Cell Signaling Technology) 1:1000 1:1000 AMPK (Cell Signaling Technology) 1:1000 1:1000 p-mTOR (Cell Signaling Technology) 1:1000 1:1000 mTOR (Cell Signaling Technology) 1:1000 1:1000 p-NFκB (Abcam) 1:1000 1:2000 RECK (Cell Signaling Technology) 1:1000 1:1000 α-SMA (Cell Signaling Technology) 1:1000 1:2000 Rabbit polyclonal antibodies ADAM-17 (GeneTex) 1:1000 1:1000 Fibronectin (Abcam) 1:1000 1:1000 GAPDH (Proteintech) 1:20000 1:6000 MIC-19 (Abcam) 1:1000 1:2000 TIMP-2 (Abcam) 1:1000 1:2000 ..

Incubation:

Article Title: Identification of RECK as a protective prognostic indicator and a tumor suppressor through regulation of the ERK/MAPK signaling pathway in gastric cancer
Article Snippet: After extracting the total protein with the Column Tissue & Cell Protein Extraction Kit (Yamei, Shanghai, China), the proteins were separated by 10% SDS-PAGE and then transferred onto polyvinylidene fluoride membranes (Thermo Fisher, Waltham, MA, USA). .. Next, the membranes were blocked with 5% skim milk for 2 h, incubated with primary antibodies at 4 °C overnight, including β-actin (Abcam, diluted 1:5000, ab6276), RECK (Cell Signalling Technology, diluted 1:1000, #3433S), CALD1 (Abcam, diluted 1:10,000, ab32330), p-CALD1 (Abcam, diluted 1:1500, ab76106, phospho S759), GAPDH (Cell Signalling Technology, diluted 1:1000, #5174), Histone (Proteintech, diluted 1:2000, #17168-1-AP), p21 (Cell Signalling Technology, diluted 1:1000, #10355-1-AP), PCNA (Cell Signalling Technology, diluted 1:20,000, #10205-2-AP), E cadherin (Cell Signalling Technology, diluted 1:1000, #14472), N cadherin (Cell Signalling Technology, diluted 1:1000, #4061S), Vimentin (Cell Signalling Technology, diluted 1:1000, #5741S), MMP2 (Cell Signalling Technology, diluted 1:1000, #87809S), p38 (Cell Signalling Technology, diluted 1:1000, #8690S), p-p38 (Cell Signalling Technology, diluted 1:1000, #4511S), JNK1/2 (Cell Signalling Technology, diluted 1:1000, #3708S), p-JNK1/2 (abcm, diluted 1:1000, ab4821), ERK (Cell Signalling Technology, diluted 1:1000, #4695S), p-ERK (Cell Signalling Technology, diluted 1:1,000, #4370S), MEK (Cell Signalling Technology, diluted 1:1000, #4694S), p-MEK (Cell Signalling Technology, diluted 1:1000, #9154S). .. Then the membranes were treated with a horseradish peroxidase-conjugated secondary antibody (Bioss, Beijing, China) for 1 h. Enhanced chemiluminescence reagents (Beyotime, Shanghai, China) were used to detect and capture images of the protein bands.

Article Title: Identification of RECK as a protective prognostic indicator and a tumor suppressor through regulation of the ERK/MAPK signaling pathway in gastric cancer.
Article Snippet: After extracting the total protein with the Column Tissue & Cell Protein Extraction Kit (Yamei, Shanghai, China), the proteins were separated by 10% SDS-PAGE and then transferred onto polyvinylidene fluoride membranes (Thermo Fisher, Waltham, MA, USA). .. Next, the membranes were blocked with 5% skim milk for 2 h, incubated with primary antibodies at 4 °C overnight, including β-actin (Abcam, diluted 1:5000, ab6276), RECK (Cell Signalling Technology, diluted 1:1000, #3433S), CALD1 (Abcam, diluted 1:10,000, ab32330), p-CALD1 (Abcam, diluted 1:1500, ab76106, phospho S759), GAPDH (Cell Signalling Technology, diluted 1:1000, #5174), Histone (Proteintech, diluted 1:2000, #17168-1-AP), p21 (Cell Signalling Technology, diluted 1:1000, #10355-1-AP), PCNA (Cell Signalling Technology, diluted 1:20,000, #10205- 2-AP), E cadherin (Cell Signalling Technology, diluted 1:1000, #14472), N cadherin (Cell Signalling Technology, diluted 1:1000, #4061S), Vimentin (Cell Signalling Technology, diluted 1:1000, #5741S), MMP2 (Cell Signalling Technology, diluted 1:1000, #87809S), p38 (Cell Signalling Technology, diluted 1:1000, #8690S), p-p38 (Cell Signalling Technology, diluted 1:1000, #4511S), JNK1/2 (Cell Signalling Technology, diluted 1:1000, #3708S), p-JNK1/2 (abcm, diluted 1:1000, ab4821), ERK (Cell Signalling Technology, diluted 1:1000, #4695S), p-ERK (Cell Signalling Technology, diluted 1:1,000, #4370S), MEK (Cell Signalling Technology, diluted 1:1000, #4694S), p-MEK (Cell Signalling Technology, diluted 1:1000, #9154S). .. Then the membranes were treated with a horseradish peroxidaseconjugated secondary antibody (Bioss, Beijing, China) for 1 h. Enhanced chemiluminescence reagents (Beyotime, Shanghai, China) were used to detect and capture images of the protein bands.

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